Summary of Study ST002547
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001640. The data can be accessed directly via it's Project DOI: 10.21228/M8QF0W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002547 |
Study Title | C57bl/6 mice subjected to either PBS or bleomycin treatment to develop fibrosis |
Study Summary | C57bl/6 mice subjected to either PBS or bleomycin treatment. By day 21, lung fibrosis will develop. We resected the lung and performed LC-QTOF analysis on small molecule metabolites between the two groups. |
Institute | University of Florida |
Last Name | Sun |
First Name | Ramon |
Address | 1200 Newell Drive, ARB, Gainesville, FL, 32610, USA |
ramonsun@ufl.edu | |
Phone | 8594733233 |
Submit Date | 2023-04-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-20 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002653 |
Sampleprep Summary: | Approximately 20mg of pulverized, frozen tissue was extracted in 3ml ice-cold 60% acetonitrile by extensively vortexing. Next, 1ml chloroform was added to each sample and samples were mixed by manual shaking followed by centrifuging at 3,200g for 20 minutes at 4°C. The aqueous layer was moved to a new tube and lyophilized. The middle layer containing protein and insoluble material was transferred to a new tube, washed twice with 50% methanol, once with 100% methanol, and briefly dried in a speed-vac. After drying, the insoluble material was hydrolyzed by heating samples in 3N HCl at 95°C for 2 hours. 100% methanol was added to hydrolysate to achieve a final concentration of 50% methanol, samples mixed, centrifuged at 18,000g for 10 min at 4°C, then supernatant dried on a speed vac. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |