Summary of Study ST002791
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001739. The data can be accessed directly via it's Project DOI: 10.21228/M8XD9T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002791 |
| Study Title | Nucleotide metabolism in pancreatic cancer cells |
| Study Summary | The Experiment analyzes the cancer cell metabolism in two pancreatic cancer cell lines, (i.e. Panc02 and KPC FC 1245) after the knockdown of the protein cytidine deaminase (CDA). Murine Panc02 and KPC FC1245 pancreatic cancer cell lines were genetically engineered using a doxycycline inducible CRISPR/Cas9 platform with a target specific gRNA for CDA and a control non-targeting NT gRNA. CDA knockdown cells show a significant decrease of intracellular uridine levels and the accumulation of intracellular cytidine. Consistent with the decrease in intracellular uridine, sgCda cells show reduced intracellular levels of UMP, UDP and UTP compared to sgNT cells while we see no change in adenine and cytosine nucleotides (i.e., AMP, ADP, ATP and CMP, CDP, CTP, respectively) or in UDP-hexose. |
| Institute | VIB-KU Leuven |
| Last Name | Mazzone |
| First Name | Massimiliano |
| Address | Campus Gasthuisberg Herestraat 49, box 912 B-3000 Leuven Belgium |
| massimiliano.mazzone@kuleuven.vib.be | |
| Phone | +32-16-37.32.13 |
| Submit Date | 2023-07-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-01-24 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002904 |
| Sampleprep Summary: | The samples were centrifuged at 20.000 x g for 15 min at 4°C, supernatant was transferred to appropriate M/S vial for analysis. The cell pellet was dissolved in 100 μl of 200 mM NaOH (for 20 minutes at 95°C), and the protein concentration was determined by using bicinchoninic acid (BCA) reagent method. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
