Summary of Study ST002912
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001773. The data can be accessed directly via it's Project DOI: 10.21228/M8J420 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002912 |
Study Title | Polar metabolite levels in MEL cells following folate depletion |
Study Summary | Culture of MEL cells in RPMI media containing 100 nM folic acid for up to 8 days followed my metabolomics targeting polar metabolites. All samples were harvested at the same time and the study was set up as a reverse time-course. Day 0 samples were cultured in 2,000 nM folate for the full 8 days. |
Institute | Boston Children's Hospital, Harvard Medical School |
Department | pathology |
Laboratory | Kanarek Lab |
Last Name | Kanarek |
First Name | Naama |
Address | Enders 1116.2, 300 Longwood Ave, Boston, MA 02115 |
naama.kanarek@childrens.harvard.edu | |
Phone | 6173557433 |
Submit Date | 2023-10-01 |
Num Groups | 6 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-02-13 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003031 |
Sampleprep Summary: | Pellet was resuspended in extraction buffer (80% Methanol, 25 mM Ammonium Acetate and 2.5 mM Na-Ascorbate prepared in LC-MS water, supplemented with isotopically-labelled amino acid standards [Cambridge Isotope Laboratories, MSK-A2-1.2], aminopterin, and reduced glutathione standard [Cambridge Isotope Laboratories, CNLM-6245-10]). Samples were vortexed for 10 sec, then centrifuged for 10 minutes at 18,000 g to pellet cell debris. The supernatant was divided into two tubes and dried on ice using a liquid nitrogen dryer. One tube of dried sample was used for polar metabolite detection. Dried samples were resuspended in 25 μL water |