Summary of Study ST002957
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001837. The data can be accessed directly via it's Project DOI: 10.21228/M88B0T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002957 |
Study Title | Metabolite flux from temperature-acclimated diatom strains (main experiment) |
Study Summary | The temperature increase occurring in the surface ocean has fundamental implications for physiological rates and processes of marine microbes. Here we asked whether the temperature at which a marine diatom strain is acclimated affects carbon transfer to a co-cultured heterotrophic bacterium. Model systems were established in which the diatom Thalassiosira pseudonana was acclimated for three months at temperatures below (14°C), equal to (20°C), and above (28°C) the temperature of optimal growth, and then inoculated with the heterotrophic bacterium Ruegeria pomeroyi. This deposition is for the results of diatom endometabolites obtained from the main experiment of this study. |
Institute | University of Georgia |
Laboratory | Moran Lab, Edison Lab |
Last Name | Uchimiya |
First Name | Mario |
Address | 315 Riverbend Rd, Athens, GA, 30602, USA |
mario.uchimiya@uga.edu | |
Phone | (706) 542-8387 |
Submit Date | 2023-10-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2023-11-23 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP003076 |
Sampleprep Summary: | Filter samples were transferred into 15-mL of ultrapure MilliQ water in 50-mL tubes, and diatom cells were removed from the filters by sonication in an ice-water bath for 7 min (cycle: 50 s on and 10 s off). The liquid fraction was subsequently collected in new tubes and the procedure repeated three times, after which fractions were combined and stored at -80°C until further processing. Samples were lyophilized (Labconco, Kansas City, MO, USA) and pellets mixed with 600 μL of phosphate buffer (30 mmol L-1 phosphate in deuterated water, pH 7.4) and 1 mmol L-1 internal standard 2,2-dimethyl-2-silapentane-5-sulfonate (DSS). Samples were vortexed for 5 min, centrifuged at 20,800 relative centrifugal force (RCF) for 10 min, and supernatants were transferred to 5-mm NMR tubes (Bruker, Billerica, MA, USA). Extraction and buffer blank controls were also prepared. Additionally, one pooled control sample was prepared by combining aliquots of all the samples and used for annotation. |
Sampleprep Protocol Filename: | 4_Sample preparation protocol_UGA_temp_Oct2023_main.docx |