Summary of Study ST003026

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001880. The data can be accessed directly via it's Project DOI: 10.21228/M8Q72K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003026
Study Title	Untargeted Metabolomics Reveals Unique Biomolecular Signatures in Overweight and Obesity Using UHPLC-ESI-QTOF-MS Analysis
Study Type	LC/MS/MS
Study Summary	Aims: Obesity poses a multifaceted challenge to global public health, impacting individuals and society in various ways. Apart from the heightened susceptibility to chronic conditions such as diabetes, cardiovascular diseases, obesity significantly escalates healthcare costs. Effective public health strategies are essential for addressing issues related to early detection, diagnosis, and personalized treatment plans. This emphasizes the crucial need for a deep understanding of biochemical pathways, patient monitoring, and prognosis. In this context, metabolomics has become a valuable approach, focusing on the identification of metabolites in biofluids and tissues. Main Methods: In this study, an untargeted metabolomics-based method was employed to investigate metabolomic changes and their relationship to pathways in overweight and obese individuals. Plasma samples were collected from 29 healthy individuals with normal weight, 17 overweight individuals, and 28 obese individuals who met the inclusion criteria for the study. The plasma samples were analyzed using highly sensitive ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight mass spectrometry. Results: Pantothenic acid and L-proline showed increased levels in the overweight group, whereas phenylacetaldehyde and glycerophosphocholine were notably decreased compared to the normal weight group. Conversely, the obese group exhibited elevated levels of specific metabolites, including L-leucine, L-tryptophan, phenylalanine, and tyrosine. On the contrary, the obese group demonstrated decreased levels of other metabolites such as 2,3-Diaminopropionic acid, and phenylacetaldehyde. Additionally, significant changes in metabolic pathways, such as pantothenate and CoA biosynthesis, and beta-alanine metabolism, were observed in the overweight group. In contrast, the obese group displayed significant alterations in phenylalanine and tyrosine metabolism, tryptophan metabolism, and beta oxidation of very long-chain fatty acids. Conclusion: The present investigation sheds light on the potential diagnostic significance of certain metabolites in obesity and the impact of their level changes on specific metabolic pathways. Additional studies are necessary to confirm the association of these metabolites in obesity and to confirm their diagnostic value.
Institute	Sharjah Institute for Medical Research
Department	Research institute of medical and health science
Laboratory	Biomarker Discovery Group
Last Name	Facility
First Name	Core
Address	M32, SIMR, College of Pharmacy, Health Sciences, University of Sharjah, Sharjah, UAE, Sharjah, 000, United Arab Emirates
Email	tims-tof@sharjah.ac.ae
Phone	+971 6 5057656
Submit Date	2023-12-25
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2024-05-28
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003146
Sampleprep Summary:	Upon aliquoting the samples into 100 µl Eppendorf tubes, 300 µl of methanol (sourced from Wunstorfer Strasse, Seelze, Germany) was introduced. The tubes underwent thorough mixing with a vortex mixer and were subsequently incubated at –20 °C for 2 hours. After this period, the samples were vortexed again and centrifuged for 15 minutes at 14,000 rpm. The resulting supernatant underwent evaporation at 35–40 °C. To guarantee the analysis's consistency and reliability, a quality control (QC) sample was prepared by combining an equal volume (10 µl) from each individual sample. This QC sample was injected into the system after every 9-10 samples to evaluate the analysis's reproducibility. Before injection, the extracted samples were reconstituted in 250 µl of 0.1% formic acid in deionized water, using Honeywell's LC-MS CHROMASOLV, situated in Wunstorfer Strasse, Seelze, Germany. Following the completion of sample preparation, the supernatant underwent filtration for subsequent LC-MS/MS analysis. This filtration utilized a hydrophilic nylon syringe filter with a pore size of 0.45 µm. The filtered sample was meticulously collected within a specialized insert positioned inside LC glass vials, ensuring its integrity for further analysis.

Sampleprep ID:

SP003146

Sampleprep Summary:

Upon aliquoting the samples into 100 µl Eppendorf tubes, 300 µl of methanol (sourced from Wunstorfer Strasse, Seelze, Germany) was introduced. The tubes underwent thorough mixing with a vortex mixer and were subsequently incubated at –20 °C for 2 hours. After this period, the samples were vortexed again and centrifuged for 15 minutes at 14,000 rpm. The resulting supernatant underwent evaporation at 35–40 °C. To guarantee the analysis's consistency and reliability, a quality control (QC) sample was prepared by combining an equal volume (10 µl) from each individual sample. This QC sample was injected into the system after every 9-10 samples to evaluate the analysis's reproducibility. Before injection, the extracted samples were reconstituted in 250 µl of 0.1% formic acid in deionized water, using Honeywell's LC-MS CHROMASOLV, situated in Wunstorfer Strasse, Seelze, Germany. Following the completion of sample preparation, the supernatant underwent filtration for subsequent LC-MS/MS analysis. This filtration utilized a hydrophilic nylon syringe filter with a pore size of 0.45 µm. The filtered sample was meticulously collected within a specialized insert positioned inside LC glass vials, ensuring its integrity for further analysis.