Summary of Study ST003104
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001927. The data can be accessed directly via it's Project DOI: 10.21228/M8N42X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003104 |
Study Title | Metabolomics studies on human cardiac samples |
Study Summary | Targeted metabolomics was performed to measure polar metabolites in both positive and negative ionization mode on left ventricular tissue acquired from pre-mortem healthy donor hearts as classified by formal pathological examination and stored at the Sydney Heart Bank. The minimum number of observations for young (age ≤ 25 years) and old (age ≥ 50 years) cohorts using liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
Institute | University of Sydney |
Department | Medicine and Health |
Last Name | Koay |
First Name | Yen Chin |
Address | Charles Perkin Centre |
yen.koay@sydney.edu.au | |
Phone | +61486275851 |
Submit Date | 2023-09-10 |
Num Groups | 2 |
Total Subjects | 25 |
Num Males | 15 |
Num Females | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | API |
Release Date | 2024-03-11 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003225 |
Sampleprep Summary: | Briefly, approximately 50 mg of ground heart tissue was weighed into 2 mL Eppendorf tube and subjected to a two-phase extraction protocol involving tissue, ice-cold extraction medium (methanol:water, and chloroform). Samples were then centrifuged at 14 300 rpm at 4 °C for 25 min. The aqueous layer was transferred into a new microfuge tube, concentrated in the Speed-Vac SPD120 (Thermo Fisher Scientific) and dried under nitrogen stream, followed by reconstitution in the acetonitrile/methanol/formic acid (75:25:0.2; v/v/v, HPLC grade; Thermo Fisher Scientific) for the HILIC analysis, and acetonitrile/methanol (25:25; v/v/v, HPLC grade) for the AMIDE analysis. |