Summary of Study ST003203

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001997. The data can be accessed directly via it's Project DOI: 10.21228/M8KT6Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST003203
Study Title	Metabolomic investigation of tear fluid biomarkers obtained from participants enrolled in the BiZiFED nutrition study
Study Summary	Zinc is an essential micronutrient, and its deficiency is particularly prevalent amongst the female population of Pakistan, leading to a substantial health burden on women of reproductive age and their children. Biofortification of wheat with zinc may offer a cost-effective strategy to attempt to improve nutritional status on a national scale. Biofortification with Zinc and Iron for Eliminating Deficiency (BiZiFED) is research program that aims to evaluate the impact of consuming zinc biofortified wheat flour on the zinc status and associated health outcomes of vulnerable communities northwest Pakistan. Of the health outcomes measured, tear biofluid was collected as a non-invasive method of determining whether zinc supplementation altered tear metabolomic biomarkers. In a crossover trial, 25 women of reproductive age received standard, non-biofortified wheat flour, and another 25 women received zinc-biofortified wheat flour for 8 weeks. At the end of this period, the nutritional intervention was switched for a further 8 weeks, whereby those individuals receiving the zinc-biofortified flour subsequently received the standard flour, whilst those receiving standard flour subsequently received the zinc-biofortified flour. The tear biofluid was collected using Schirmer strips after a 2-week baseline period and after 8 weeks and 16 weeks of the study. Metabolomic analysis was conducted using the MxP Quant 500 kit (Biocrates). Following multiple comparisons, four metabolites were significantly correlated with plasma zinc concentration, two demonstrating a positive correlation and two demonstrating a negative correlation. Five metabolites were significantly altered in response to zinc supplementation with biofortified wheat flour. These results highlight the potential of using tear biofluids as an alternative source for metabolomic biomarkers for the assessment of zinc status of individuals enrolled in nutritional studies.
Institute	Institute for Global Food Security
Last Name	Pan
First Name	Xiaobei
Address	19 Chlorine Gardens, Belfast, Antrim, BT9 5DL, United Kingdom
Email	x.pan@qub.ac.uk
Phone	02890975984
Submit Date	2024-04-22
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2024-05-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003329
Sampleprep Summary:	One Schirmer strip from each participant at each timepoint was cut into 5 mm sections, up until the tear front was reached, and placed into sterile tubes. A length-adjusted volume of ice-cold 80% methanol was then added to each tube (500 µL per 46 mm Schirmer strip). Strips were vigorously vortexed in the extraction solution for 2 mins before centrifugation at 15,700 RCF at 4°C. Supernatant was transferred to a fresh, clean tube and stored at -80°C before analysis. Targeted metabolomics profiling was performed using a commercially available kit, MxP® Quant500 kit as instructed.

Sampleprep ID:

SP003329

Sampleprep Summary:

One Schirmer strip from each participant at each timepoint was cut into 5 mm sections, up until the tear front was reached, and placed into sterile tubes. A length-adjusted volume of ice-cold 80% methanol was then added to each tube (500 µL per 46 mm Schirmer strip). Strips were vigorously vortexed in the extraction solution for 2 mins before centrifugation at 15,700 RCF at 4°C. Supernatant was transferred to a fresh, clean tube and stored at -80°C before analysis. Targeted metabolomics profiling was performed using a commercially available kit, MxP® Quant500 kit as instructed.