Summary of Study ST003243
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003243 |
Study Title | Lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
Study Summary | The rate-limiting enzyme in glutathione (GSH) synthesis is Gclc. GSH synthesis is suggested to be implicated in lipogenesis. Loss of GSH synthesis is suggested to increase the activity of NRF2. To examine the impact of GSH and NRF2 on lipid abundance in serum, we performed lipidomic analysis of serum from WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO mice. |
Institute | University of Rochester Medical Center |
Last Name | Harris |
First Name | Isaac |
Address | 601 Elmwood Ave, Rochester, New York, 14642-0001, USA |
isaac_harris@urmc.rochester.edu | |
Phone | 8572348624 |
Submit Date | 2024-05-29 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-06-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003369 |
Sampleprep Summary: | For serum for WT, liver-specific Gclc KO, liver-specific Nrf2 KO, and liver-specific Gclc-Nrf2 DKO, 20 µL of mouse serum was combined with 180 µL of chloroform:methanol extraction solvent (v:v=1:2) containing internal standards at the final concentrations: 5nM D7-Sphinganine (Avanti Polar Lipids Inc., Cat# 860658), 12.5nM D3-Deoxysphinganine (Avanti Polar Lipids Inc., Cat# 860474), and SPLASH LIPIDOMIX (1:1000, Avanti Polar Lipids Inc., Cat# 330707). After sonicating (1400 rpm, 20°C, 5 min), the extracts were cleared by centrifugation (17,000g, 20°C, 10 min), and the lipids in the supernatant were analyzed by LC-MS. |