Summary of Study ST003265
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002027. The data can be accessed directly via it's Project DOI: 10.21228/M8M24P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003265 |
| Study Title | Malic Enzyme 2 maintains metabolic state and anti-tumor immunity of CD8+ T cells |
| Study Summary | For metabolite analysis, naive ME2+/+ and ME2-/- CD8+ T cells were isolated and activated with anti-CD3 (4μg/ml) and anti-CD28 (1μg/ml). Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. The extracts were analyzed by LC-MS/MS. |
| Institute | Peking Union Medical College |
| Last Name | zhang |
| First Name | zhenxi |
| Address | Beijing Dongdan Santiao No.5 |
| 13121716766@163.com | |
| Phone | 69156953 |
| Submit Date | 2024-06-05 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-07-15 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003392 |
| Sampleprep Summary: | For metabolite analysis, naive ME2+/+ and ME2-/- CD8+ T cells were isolated and activated with anti-CD3 (4μg/ml) and anti-CD28 (1μg/ml). Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. The extracts were analyzed by LC-MS/MS. |
| Extraction Method: | Cells were washed twice with ice-cold PBS and metabolites were extracted with ice-cold 80% methanol. |
| Extract Storage: | -80℃ |
