Summary of Study ST001042
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000698. The data can be accessed directly via it's Project DOI: 10.21228/M8FT3B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001042 |
Study Title | Non-targeted serum metabolomic profiling of prostate cancer patients (FI-TWIM-MS PCa ) |
Study Summary | Non-targeted metabolomics study of serum samples from prostate cancer and healthy individuals using flow injection ion mobility mass spectrometry |
Institute | Georgia Institute of Technology |
Last Name | Zang |
First Name | Xiaoling |
Address | 901 Atlantic Drive Atlanta, GA 30332-0400 |
xzang3@gatech.edu | |
Phone | 4043850381 |
Submit Date | 2018-06-07 |
Analysis Type Detail | FIA-MS |
Release Date | 2019-07-17 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001088 |
Sampleprep Summary: | Frozen serum samples were thawed on ice, and 300 µL of extraction solution (acetone: acetonitrile: methanol 1:1:1, cooled to -20 ˚C) was added to 100 µL of serum. Samples were vortex-mixed for 20 s and centrifuged at 16,000 × g for 5 min to pellet proteins. To remove most lipids and other non-polar metabolites, 800 µL of dichloromethane was added to ~350 µL of supernatant and vortex-mixed. Following the addition of 250 µL deionized water, samples were vortex-mixed and kept on ice for 10 min. The aqueous phase was subject to FI-TWIM-MS analysis. Sample blanks were prepared using deionized water instead of serum following the same procedure. |