Summary of Study ST001090
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000729. The data can be accessed directly via it's Project DOI: 10.21228/M8FM38 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001090 |
Study Title | NMR metabolomics study of Gemcitabine resistant cancer cells |
Study Type | NMR metabolomics |
Study Summary | 1D 1H NMR spectra were collected from the samples and analyzed using MVAPACK software (http://bionmr.unl.edu/mvapack.php). Multivariate analysis was conducted to study the metabolic phenotypes of the samples. |
Institute | University of Nebraska-Lincoln |
Department | Chemistry |
Laboratory | Dr. Robert Powers and Pankaj Singh labs |
Last Name | Gebregiworgis |
First Name | Teklab |
Address | 552 Hamilton Hall, 639 N. 12th Street, Lincoln, NE, 68588, USA |
teklab@huskers.unl.edu | |
Phone | (402) 472-3039 |
Submit Date | 2018-09-25 |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2019-10-11 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001141 |
Sampleprep Summary: | The dried metabolite extracts were reconstituted in 600 µL of 50 mM phosphate buffer in 99.8% D2O (Isotec, St. Louis, MO) at pH 7.2 (uncorrected). 50 µM of TMSP (3-(tetramethysilane) propionic acid-2,2,3,3-d4) were added for spectral referencing. The solution was mixed by gentle votexing and centrifuged at 13000 rpm for 5 min, and then the supernatant were transferred to 5 mm NMR tube. |