Summary of Study ST001155

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000774. The data can be accessed directly via it's Project DOI: 10.21228/M8N096 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001155
Study TitleThe Effect of Silicon on Salinity Tolerance and the Associated Metabolomics Profile Changes in Date Palm
Study SummarySilicon has a promising role in the growth and salinity tolerance in plants. While the results obtained from the current study showed that silicon enhanced growth in date palm seedlings, the mechanism behind this observation was also investigated by studying changes occurred in metabolomic profiles triggered by silicon under salinity. The global metabolomic analysis using liquid-chromatography-mass-spectrometry revealed the presence of a number significantly (p ≤ 0.05) accumulated metabolites in leaves and roots when plants were irrigated with silicon and grown under control and salinity conditions.
Institute
Sultan Qaboos University
DepartmentBiology
Last NameYaish
First NameMahmoud
AddressSultan Qaboos University, Department of Biology, College of Science
Emailmack.yaish@gmail.com
Phone968 24146823
Submit Date2019-03-18
Raw Data AvailableYes
Raw Data File Type(s)metdb
Analysis Type DetailLC-MS
Release Date2020-03-18
Release Version1
Mahmoud Yaish Mahmoud Yaish
https://dx.doi.org/10.21228/M8N096
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001228
Sampleprep Summary:A total of 24 samples were analyzed using LC-MS by HILIC chromatography on a 0.5 x 150 mm Zic-pHILIC column using 10 mM NH4HCO3 in water (A) and 10 mM NH4HCO3 in 95% acetonitrile (B) as solvents and by RPLC chromatography using the same solvents and a 0.5 x 100 mm PLRPS column. The Samples were extracted at a tissue concentration of 100 mg/mL (leaves) or 50 mg/mL (roots) in 80% methanol by vortexing for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.
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