Summary of Study ST001170
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000782. The data can be accessed directly via it's Project DOI: 10.21228/M8M10T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001170 |
Study Title | Timecourse on MCF-7 cells treated with different concentration of doxorubicin |
Study Summary | MCF-7 cells treated with 10μM doxorubicin for 4h followed by subsequent withdrawl of the drug and cultured up to 48h.Doxurubicin-treated cells and control cells were collected at 0,12,24,36,and 48h. Meanwhile,MCF-7 cells continuously exposed to a low dosage of doxorubicin at 0.1μM for 96h. Doxorubicin-treated cells and control cells were collected at 0,24,48,72 and 96h. |
Institute | China Pharmaceutical University |
Last Name | Shao |
First Name | Chang |
Address | Tongjiaxiang #24, Nanjing, Jiangsu, 210000, China |
cici_shao@126.com | |
Phone | 13951628679 |
Submit Date | 2019-04-11 |
Raw Data Available | Yes |
Raw Data File Type(s) | qgd |
Analysis Type Detail | LC-MS |
Release Date | 2020-12-01 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001243 |
Sampleprep Summary: | MCF-7 cells were harvested and permeabilized with cold (80% (v/v) methanol: water solution followed by a 20-min incubation at -80 ℃. A stock solution of 4-Chloro-DL-phenylalanine was pre-spiked into the methanol aqueous buffer to a final concentration of 1.5 μg/mL, which subsequently served as an internal standard (IS). The cell lysates were centrifuged at 14,000g for 5 min at 4 ℃, and the resultant supernatant were collected and evaporated to dryness. The samples were reconstituted in 100 μL of water (LC-MS grade) before analysis on an HPLC−MS/MS, and 20 μL was used for analysis. For each group of samples subjected to metabolomics analysis, five biological replicates were prepared. |