Summary of Study ST001277
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000862. The data can be accessed directly via it's Project DOI: 10.21228/M8896R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001277 |
Study Title | Pseudoexfoliation Aqueous Humor Metabolites from Veterans Affairs Patients |
Study Summary | We performed high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) Isotopic Ratio Outlier Analysis of aqueous humor samples from patients from patients with pseudoefoliation syndrome (PEX). |
Institute | University of Miami |
Department | Ophthalmology, Bascom Palmer Eye Institute |
Laboratory | Sanjoy K. Bhattacharya |
Last Name | Bhattacharya |
First Name | Sanjoy |
Address | 1638 NW 10th Avenue, Miami, FL 33136 |
SBhattacharya@med.miami.edu | |
Phone | 305-482-4103 |
Submit Date | 2019-11-14 |
Num Groups | 1 |
Total Subjects | 31 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2020-05-14 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001357 |
Sampleprep Summary: | The 31 samples were prepared for isotopic ratio outlier analysis (IROA). Approximately 800 µL of precipitate solution (8:1:1 Acetonitrile: Methanol: Acetone) was added to the 50 µL of aqueous humor. The metabolites were vortexed and incubated at 4 °C for 30 minutes. This was followed by another incubation period at -20 °C for 1 hour. Each sample was centrifuged at 20,000 x g for 10 minutes at 4 °C (Beckman Microfuge 18) to form a pellet and 375 µL of supernatant was collected. The supernatant was dried in a speed vacuum for approximately 20 minutes or until the sample was fully dry and stored at -20 °C. The IROA internal standard (IROA-IS, IROA technologies) (U-95% 13C) was reconstituted in 1.2 mL of LC-MS grade water. The samples were reconstituted in 25 µL of LC-MS grade water and 10 µL was combined with 20 µL of IROA-IS and subjected to LC-MS/MS analysis. A long-term reference standard (LTRS) was reconstituted in 50 µL of LC-MS grade water and subjected to LC-MS/MS as the first, last, and every 10 samples. |