Summary of Study ST001371
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000937. The data can be accessed directly via it's Project DOI: 10.21228/M8K97X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001371 |
Study Title | Untargeted metabolomics of Quercus ilex acorns |
Study Type | LC-MSE analysis |
Study Summary | UPLC-MSE analysis of samples from Quercus ilex acorns flour. The objective of the study is to obtain a metabolomic profile of several acorns from different trees. This phytochemical analysis and characterization will be a base for the identification of bioactive, antinutritional, or toxic compounds and traceability analysis. |
Institute | Universidad de Córdoba |
Department | Department Biochemistry and Molecular Biology |
Laboratory | Agroforestry and Plant Biochemistry, Proteomics and Systems Biology |
Last Name | López-Hidalgo |
First Name | Cristina |
Address | Campus de Rabanales; Edificio C6, Planta Baja |
n12lohic@uco.es | |
Phone | 626894948 |
Submit Date | 2020-04-25 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2020-06-01 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001453 |
Sampleprep Summary: | Metabolites were extracted from acorn flour as described by Valledor et al., (2014), with minor modifications. 600 µL of ice-cold methanol: chloroform: water (5:2:2) was added to 50 mg of acorn flour, mixed by vortexing, and the mixture sonicated (ultrasonic bath, 40 kHZ for 10 min). After centrifugation (4 oC, 4 min, 20,000 × g), the pellet was once more extracted with 200 µL of cold methanol: chloroform: water (5:2:2). The two supernatants were combined and vacuum dried at 30 oC (Speedvac, Eppendorf Vacuum Concentrator Plus/5301). Dried extracts were reconstituted in methanol, centrifuged at 20,000 × g for 10 min, filtered through 0.22 µm PTPE membranes (Thermo Scientific, MA, USA) and filtrate collected in 1.5 mL LC/MS certified sample vials. |
Processing Storage Conditions: | Room temperature |
Extract Storage: | On ice |
Sample Resuspension: | Methanol |