Summary of Study ST001634
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001045. The data can be accessed directly via it's Project DOI: 10.21228/M8MQ3V This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001634 |
Study Title | A combinatorial action of GmMYB176 and bZIP controls isoflavonoid biosynthesis in soybean. |
Study Summary | This study identified how the GmMYB176 protein complex affects the metabolome of soybean hairy roots using non-targeted high resolution mass spectrometry. |
Institute | Agriculture and Agri-Food Canada |
Last Name | Renaud |
First Name | Justin |
Address | 1391 Sandford Street, London, Ontario, N5V 4T3, Canada |
justin.renaud@canada.ca | |
Phone | 519-953-6698 |
Submit Date | 2020-12-17 |
Num Groups | 2 |
Total Subjects | 10 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2021-01-07 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001717 |
Sampleprep Summary: | For metabolomics analysis, frozen hairy roots were ground with liquid nitrogen and extracted in methanol:water (80:20, v/v). The samples were sonicated on ice water bath for 15 min followed by centrifugation at 11,000×g for 10 min at ambient temperature. The supernatant (350 µL) was dried under nitrogen gas. The dried pellet was dissolved in 200 µL of 50% methanol containing 10 µg caffeine as an internal standard and filtered through a 0.45 µm syringe filter. |
Sampleprep Protocol ID: | jrenaud_SP_Sample_preparation.pdf |