Summary of Study ST001776
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001130. The data can be accessed directly via it's Project DOI: 10.21228/M8N706 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001776 |
Study Title | Metabolic Response of HEK 293 Cells Exposed to Methylmercury |
Study Summary | HEK 293 cells were treated with 7.5 μM methylmercury (HgMe) for 48 h. Metabolites were extracted and subjected to LC-HRMS based metabolomics. LC-HRMS data were processed by SIEVE2.2 software. Metabolites associated with HgMe toxicity were screened and identified. |
Institute | University of Macau |
Last Name | Zhang |
First Name | pw |
Address | Taipa, Macau SAR, China |
yb47620@um.edu.mo | |
Phone | 8613924251358 |
Submit Date | 2021-04-27 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2022-01-02 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP001859 |
Sampleprep Summary: | cells were washed gently with 150 mM ammonium acetate buffer and scraped quickly with a rubber-tipped cell scraper. Subsequently, 500 uL chilled 80% methanol was added and the scraped cells were sonicated using a bioruptor (Diagenode, Philadelphia, PA, USA) in 4 °C water bath during 10 cycles (30s on and 30s off). After that, the sample was placed at -20 °C for 3 h, followed by 10 min of centrifugation at 14000 g at 4 °C. The supernatant containing the metabolite was dried by speedvac and stored at -80 °C until LC-HRMS analysis.The residual protein pellet was dissolved in protein extraction buffer and quantified for normalization. |
Processing Storage Conditions: | 4℃ |
Extract Storage: | -80℃ |
Sample Resuspension: | Sampe were reconstituted in 50% ACN. The volume was adjusted by the protein concentration. |