Summary of Study ST001857
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001171. The data can be accessed directly via it's Project DOI: 10.21228/M8BM4Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001857 |
Study Title | Peak Learning of Mass Spectrometry Imaging Data Using Artificial Neural Networks (Prostate tissue) |
Study Summary | The human prostate tissue sample was analyzed by 2D MALDI FT ICR MSI. For detailed information we refer to the msiPL manuscript by Abdelmoula et al. |
Institute | Brigham and Women's Hospital |
Last Name | Abdelmoula |
First Name | Walid |
Address | 60 Fenwood RD, Boston, MA |
wahassan@bwh.harvard.edu | |
Phone | 8572149765 |
Submit Date | 2021-06-20 |
Publications | https://www.nature.com/articles/s41467-021-25744-8 |
Raw Data Available | Yes |
Raw Data File Type(s) | h5 |
Analysis Type Detail | MALDI-MS |
Release Date | 2021-07-18 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001940 |
Sampleprep Summary: | As stated in the msiPL paper: For the Prostate tissue: Briefly, 12 µm thickness prostate sample diagnosed with a Gleason score of (3+4)=7 were mounted on a microscopy glass slide and coated with CHCA (5 mg/mL in 70/30 methanol/water with 0.1% trifluoroacetic acid V/V) using an automated sprayer (TM-Sprayer, HTX Imaging, Carrboro, NC). The analysis of the samples was performed on a 9.4 Tesla SolariX XR FT ICR mass spectrometer (Bruker Daltonics, Billerica, MA) using the MALDI source in positive ion mode in the mass range between 250-1000 m/z, with a spatial resolution of 120 µm. For the PDX GBM mouse brain dataset: The intracranial tumor belonging to a PDX model of GBM12 (PDX National Resource, Mayo Clinic), was analyzed by MALDI FT ICR MSI using a 9.4 Tesla SolariX mass spectrometer (Bruker Daltonics, Billerica, MA), using continuous accumulation of selected ions in the mass range between 380-620 m/z. The indium tin oxide (ITO)-coated slide with 12 µm thickness tissue sections , was coated with DHB (160 mg/mL in a 70/30 v/v solution of methanol/0.2% TFA), according to Randall et al2. The 3D MSI dataset was collected from 4 tissue sections with an inter-slice distance of 160 µm. Internal online calibration was performed using heme m/z 616.1776 during data acquisition. |