Summary of Study ST001925

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001215. The data can be accessed directly via it's Project DOI: 10.21228/M8NQ5X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001925
Study TitleMS Untargeted Metabolomics from Human Serum
Study SummaryMultiple Myeloma patients were treated with the proteasome inhibitor carfilzomib in different protocols according to their clinical stage. Paired serum samples were obtained before i.v injection and after 1-8h post treatment and snap frozen. Samples were stored at -80°C until analysis.
Institute
University of Zurich
Last NameMendez
First NameMax
AddressRorschacherstrasse 95. 9007, St.Gallen
Emailmaxmendez@runbox.com
Phone+41 71 494 10 67
Submit Date2021-09-21
Num Groups2
Total Subjects6
Num Males5
Num Females1
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2022-01-02
Release Version1
Max Mendez Max Mendez
https://dx.doi.org/10.21228/M8NQ5X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP002009
Sampleprep Summary:No. Step 1 Take 40 μL of each sample into the 96-well plate; 2 Insert fetal bovine serum (more than 50, insert one for every 10 samples; 50 or less are not considered); 3 Add 120 μL of pre-cooled methanol, mix and shake for 1 min, and place in a refrigerator at -20°C for 2h or overnight; 4 4,000g * 4°C centrifugation for 30 min; 5 Place 25 μL of each sample in a new 96-well plate and dilute with 225 μL of 50% methanol; 6 Take 50 μL of each sample and mix to QC sample; 7 Take 60 μL of the supernatant and transfer to a 96-well microplate, seal the membrane and test it on the machine.
Processing Storage Conditions:-80℃
Extract Storage:-80℃
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