Summary of Study ST001925
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001215. The data can be accessed directly via it's Project DOI: 10.21228/M8NQ5X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001925 |
Study Title | MS Untargeted Metabolomics from Human Serum |
Study Summary | Multiple Myeloma patients were treated with the proteasome inhibitor carfilzomib in different protocols according to their clinical stage. Paired serum samples were obtained before i.v injection and after 1-8h post treatment and snap frozen. Samples were stored at -80°C until analysis. |
Institute | University of Zurich |
Last Name | Mendez |
First Name | Max |
Address | Rorschacherstrasse 95. 9007, St.Gallen |
maxmendez@runbox.com | |
Phone | +41 71 494 10 67 |
Submit Date | 2021-09-21 |
Num Groups | 2 |
Total Subjects | 6 |
Num Males | 5 |
Num Females | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2022-01-02 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002009 |
Sampleprep Summary: | No. Step 1 Take 40 μL of each sample into the 96-well plate; 2 Insert fetal bovine serum (more than 50, insert one for every 10 samples; 50 or less are not considered); 3 Add 120 μL of pre-cooled methanol, mix and shake for 1 min, and place in a refrigerator at -20°C for 2h or overnight; 4 4,000g * 4°C centrifugation for 30 min; 5 Place 25 μL of each sample in a new 96-well plate and dilute with 225 μL of 50% methanol; 6 Take 50 μL of each sample and mix to QC sample; 7 Take 60 μL of the supernatant and transfer to a 96-well microplate, seal the membrane and test it on the machine. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |