Summary of Study ST001962
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001249. The data can be accessed directly via it's Project DOI: 10.21228/M88974 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001962 |
| Study Title | Nontargeted metabolomics of UGT71 CRISPR knockouts in Poplar |
| Study Summary | Non-targeted metabolomic analysis of UGT71L1 CRISPR-Cas9 knockouts in populus tremula x populus alba |
| Institute | University of Victoria |
| Last Name | Gordon |
| First Name | Harley |
| Address | 3800 Finnerty Rd |
| harleygordon@uvic.ca | |
| Phone | 2507217211 |
| Submit Date | 2021-10-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-12-01 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP002048 |
| Sampleprep Summary: | Flash frozen leaf tissue was homogenized under liquid nitrogen with mortar and pestle. Tissue was then freeze dried and stored at room temperature prior to analysis. For extraction, 50 mg of freeze-dried tissue was weighed into a 2 mL cryo-vial with 4 x 5 mm steel ball bearings and 750 mL of chilled methanol. Samples were homogenized 2 x 45 seconds at 5500 rpm using a Precellys 24 Homogenizer, then sonicated for 2 minutes in a sonicating water bath. Samples were centrifuged at 12,000 rpm for 2 minutes and the supernatant was filtered through 0.22 µm PTFE filters into glass vials. Methanol extractions were repeated twice and extracts were pooled. Extracts were dried under vacuum at ambient temperature. Final extraction weight was determined, vials were sealed and stored at -20°C until analysis. Each biological replicate included an extraction |
