Summary of Study ST002118
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001343. The data can be accessed directly via it's Project DOI: 10.21228/M8439K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002118 |
| Study Title | Exposures to Metals in Pregnant Women and the Impact on Fetal Development and Birth Outcomes in Suriname |
| Study Type | C18 Reversed-Phase Broad Spectrum Metabolomics |
| Study Summary | The Caribbean Consortium for Environmental and Occupational Health, a NIH-funded integrated research and research training program, started in 2015. The research component is a population-based prospective longitudinal environmental epidemiologic cohort study addressing the potential adverse impact of chemical and non-chemical environmental exposures in mother/child dyads in Suriname. The study determines associations between exposures to neurotoxicants (metals and pesticides) and essential elements and non-chemical stressors in pregnant women and the impact on birth and neurodevelopmental outcomes. The study population consists of culturally diverse pregnant women (n=1143; ages: 16-49 years) and their babies/children (n=1069). Data collection takes place twice prenatally, at birth, 12, 36, and 48 months. Through HHEAR, targeted and untargeted (metabolomics) analyses will characterize exposure to metals in a sub-cohort of pregnant women for whom exposure data are not yet available. This expanded exposure analysis will enable a more comprehensive cumulative risk assessment of adverse birth and neurodevelopmental outcomes in the overall cohort. |
| Institute | University of Pittsburgh |
| Department | Department of Environmental and Occupational Health |
| Last Name | Lichtveld |
| First Name | Maureen |
| Address | 130 DeSoto Street, University of Pittsburgh, Pittsburgh, PA 15261 |
| mlichtve@pitt.edu | |
| Phone | 412-624-6818 |
| Submit Date | 2022-03-22 |
| Total Subjects | 668 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-03-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002209 |
| Sampleprep Summary: | Study serum samples (50 µL) and total study pool samples (50 µL) were prepared and shipped from Tulane Univeristy to the NC HHEAR Hub on dry ice. Aliquots of HHEAR reference plasma (50 µL each) and NIST serum (909C) reference material (50 µL each) were provided by the NC HHEAR Hub. All samples were stored at -80° C until lab analysis. All samples were thawed at 4 °C overnight a day before sample preparation. The same volume (50 µL) of LC-MS grade water was used as blank. Samples, including study samples, total study pool samples, HHEAR reference material, NIST reference material, and blanks were mixed with 400 µL methanol containing 500 ng/mL tryptophan-d5 as internal standard and vortexed by a multiple tube vortex mixer for 2 min at 5000 rpm in room temperature. All samples were centrifuged at 16,000 rcf for 10 min at 4°C. The supernatant (350 µL) was transferred into a pre-labeled 2.0 mL Lo-bind Eppendorf tube, and then dried by a SpeedVac overnight. For immediate analysis, 100 µL of water-methanol solution (95:5, v/v) was used to reconstitute the dried extracts, and the samples were thoroughly mixed on a multiple tube vortex mixer for 10 min at 5000 rpm at room temperature. All samples were centrifuged at 4°C for 10 min at 16,000 rcf. The supernatant was transferred to pre-labeled autosampler vials for data acquisition by LC-MS. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Vortex with methanol containing 500ng/ml tryptophan-d5 as internal standard |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Water-Methanol (95:5, v/v) |
| Sample Spiking: | Tryptophan-d5 stock solution at 500 ng/mL |
