Summary of Study ST002183

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001390. The data can be accessed directly via it's Project DOI: 10.21228/M82117 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST002183
Study Title	Individualized exercise intervention for people with multiple myeloma improves quality of life in a randomized controlled trial
Study Summary	Although new treatments have improved survival for multiple myeloma (MM), quality of life remains poor for people with this incurable cancer. We conducted a multi-site randomized, waitlist-controlled trial of an individualized exercise program for people at all stages of MM (n=60). Compared to the waitlist control group, participants of the 12-week intervention had significant improvement in health-related quality of life, mediated through improved MM symptoms, cardiorespiratory fitness and bone pain, with were mostly maintained at follow-up (up to 12 months). Exploratory plasma metabolomics and lipidomics was conducted to delineate molecular mechanisms and biomarkers
Institute	QIMR Berghofer Medical Research Institute
Laboratory	Precision & Systems Biomedicine
Last Name	Stoll
First Name	Thomas
Address	300 Herston Road
Email	thomas.stoll@qimrberghofer.edu.au
Phone	+61 7 3845 3992
Submit Date	2022-06-01
Raw Data Available	Yes
Raw Data File Type(s)	mzML
Analysis Type Detail	LC-MS
Release Date	2024-02-05
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP002275
Sampleprep Summary:	In total, 126 human plasma samples from 46 patients were prepared. All samples (including QCs and blanks) were randomized in one batch and then evenly split between two 2 mL 96-deepwell plates (Eppendorf #0030 501.306). Human plasma samples were thawed on ice and briefly vortexed before aliquoting 100 µL plasma of each sample to a well. In addition, a global sample pool containing equal volumes (12 µL) of each sample was prepared into a 2 mL Eppendorf tube as quality control (QC) and 12 x 100 µL aliquots were transferred into wells across the 2 plates. Finally, blank negative control extraction samples were prepared by transferring 100 µL of 1X PBS to 6 wells equally across 2 plates. Ten-times the sample volume of ice-cold butanol/methanol (1:1) containing 50 µg/mL antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) and 0.5 µg/mL ISTD 4-chloro-L-phenylalanine (PCPA) was added to each well using a multi-channel pipette. Plates were covered with organic solvent resistant sealing mats (Eppendorf #0030 127.960) and vortexed for 3 min at 1000 rpm. Samples were then sonicated for 15 min in an ice-cold water bath sonicator, stored overnight at -30oC and centrifuged for 30 min at 4,000 x g (4oC). Samples were aliquoted into 96-well V-bottom plates (Greiner #651201) using a liquid handler platform (AssayMap Bravo, Agilent). From each deepwell plate, 4 x 100 µL and 2 x 200 µL aliquots were prepared, totalling 8 and 4 plates, respectively. Samples were dried down (2.5 hrs) using a Genevac EZ-2 vacuum concentrator and fast-stack swings facilitating drying of 8 plates per batch. Dried sample plates were covered with AlumaSeal CS sealing film for cold storage (Finneran-Porviar #FC-100) and stored at -80oC until LC/MS analysis

Sampleprep ID:

SP002275

Sampleprep Summary:

In total, 126 human plasma samples from 46 patients were prepared. All samples (including QCs and blanks) were randomized in one batch and then evenly split between two 2 mL 96-deepwell plates (Eppendorf #0030 501.306). Human plasma samples were thawed on ice and briefly vortexed before aliquoting 100 µL plasma of each sample to a well. In addition, a global sample pool containing equal volumes (12 µL) of each sample was prepared into a 2 mL Eppendorf tube as quality control (QC) and 12 x 100 µL aliquots were transferred into wells across the 2 plates. Finally, blank negative control extraction samples were prepared by transferring 100 µL of 1X PBS to 6 wells equally across 2 plates. Ten-times the sample volume of ice-cold butanol/methanol (1:1) containing 50 µg/mL antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) and 0.5 µg/mL ISTD 4-chloro-L-phenylalanine (PCPA) was added to each well using a multi-channel pipette. Plates were covered with organic solvent resistant sealing mats (Eppendorf #0030 127.960) and vortexed for 3 min at 1000 rpm. Samples were then sonicated for 15 min in an ice-cold water bath sonicator, stored overnight at -30oC and centrifuged for 30 min at 4,000 x g (4oC). Samples were aliquoted into 96-well V-bottom plates (Greiner #651201) using a liquid handler platform (AssayMap Bravo, Agilent). From each deepwell plate, 4 x 100 µL and 2 x 200 µL aliquots were prepared, totalling 8 and 4 plates, respectively. Samples were dried down (2.5 hrs) using a Genevac EZ-2 vacuum concentrator and fast-stack swings facilitating drying of 8 plates per batch. Dried sample plates were covered with AlumaSeal CS sealing film for cold storage (Finneran-Porviar #FC-100) and stored at -80oC until LC/MS analysis