Summary of Study ST002359
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001514. The data can be accessed directly via it's Project DOI: 10.21228/M80H6B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002359 |
Study Title | Effect of hypoxia and reoxygenation on the adult hooded seal brain lipidome |
Study Summary | Brain samples from adult hooded seals (Cystophora cristata) were subjected to hypoxia and reoxygenation in vitro and lipid composition was compared. |
Institute | University of Hamburg |
Last Name | Martens |
First Name | Gerrit Alexander |
Address | Martin-Luther-King-Platz 3, 20146 Hamburg, Germany |
gerrit.alexander.martens@uni-hamburg.de | |
Phone | +49 40 42838-3934 |
Submit Date | 2022-11-23 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | LC-MS |
Release Date | 2023-04-12 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP002454 |
Sampleprep Summary: | The extraction protocol was performed slightly modified according to the method of Bligh and Dyer (Bligh and Dyer 1959). About 20 mg sample was weighed into a 2.0 ml reaction tube (Eppendorf, Hamburg, Germany). Two steel balls (3.6 mm), 100 µl chloroform and 200 µl methanol were added to the sample. The mixture was homogenized in a ball mill (1 min, 3.1 m/s Bead Ruptor 24, Omni International IM, GA, USA)). Afterwards 200 µl water and 100 µl chloroform were added and again processed in the ball mill (1 min, 3.1 m/s). The homogenized sample was then centrifuged (20 min, 16.000xg, 5 °C, Sigma 3-16PK, Sigma, Osterode, Germany). A quality control sample (QC) was prepared by transferring 30 µl of each sample into a new vial. The organic chloroform phase was directly used for measurement. Because of limited sample number, technical triplicates of each sample were measured. |