Summary of Study ST002514
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001622. The data can be accessed directly via it's Project DOI: 10.21228/M81T5C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002514 |
| Study Title | The investigation of the role of dietary inulin in NASH progression with mouse fecal metabolites |
| Study Type | non-targeted LC-Mass analysis |
| Study Summary | To investigate the role of dietary fiber in non-alcoholic steatohepatitis (NASH) progression, male C57 mice was randomly assigned into four groups that received normal chow diet (NCD), choline deficient high fat diet (CDHFD), CDHFD + 10% inulin (CDHFD-I), CDHFD + 10% Cellulose (CDHFD-C). Additionally, some mice received inulin or cellulose was treated with 13C labelled fiber for 36 hours. Fecal metabolites were analyzed by non-targeted metabolomics.Results shown that the fecal metabolites from Inulin treatment group signficantly distinguished from that from CDHFD only group, while only negligible alterations was induced by cellulose treatment, indicating that inulin not cellulose could be well fermented by gut microbiota. The 13C tracing results shown that nineteen 13C-inulin labelled metabolites were also captured including pantothenate, phosphoethanolamine and adenosine. These metabolites are reported to play a protective role in reducing fat accumulation and ameliorating cellular oxidative stress and inflammation, indicating that the mechanism inulin suppresses NASH may through mediating modulating gut metabolites. |
| Institute | The Chinese University of Hong Kong |
| Last Name | Wei |
| First Name | Wenchao |
| Address | Prince of Wales Hospital |
| 1155118538@link.cuhk.edu.hk | |
| Phone | 56001913 |
| Submit Date | 2023-03-17 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP002620 |
| Sampleprep Summary: | 25 mg of sample was weighted to an EP tube, and 500 μL extract solution (acetonitrile: methanol: water = 2: 2: 1) containing internal standard (L-2-Chlorophenylalanine, 4 μg/mL) was added. After 30 s vortex, the samples were homogenized at 35 Hz for 4 min and sonicated for 5 min in ice-water bath. The homogenization and sonication cycle was repeated for 2 times. Then the samples were incubated at -40 ℃ for 1 h and centrifuged at 12000 rpm for 15 min at 4 ℃. 400 μL of supernatant was transferred to a fresh tube and dried in a vacuum concentrator at 37 ℃. Then, the dried samples were reconstituted in 200 μL of 50% acetonitrile by sonication on ice for 10 min. The constitution was then centrifuged at 13000 rpm for 15 min at 4 ℃, and 75 μL of supernatant was transferred to a fresh glass vial for LC/MS analysis. The quality control (QC) sample was prepared by mixing an equal aliquot of the supernatants from all of the samples. |
| Sampleprep Protocol Filename: | Protocol_for_The_role_of_fiber_in_NASH_progression_stool.docx |
