Summary of Study ST002982
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001307. The data can be accessed directly via it's Project DOI: 10.21228/M8S124 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002982 |
| Study Title | Metabolic characterization of the polar endometabolome of Triple-Negative Breast Cancer parental and cDDP-resistant cells (part 2) |
| Study Type | NMR-based metabolomics of polar endometabolome of cultured cells |
| Study Summary | Platinum (Pt(II)) drugs, e.g. cisplatin (cDDP), are some of the most used chemotherapeutic agents, yet tumor acquired resistance and high toxicity are still current drawbacks. Metabolomics can measure the metabolic response of drug-exposed cells, unveiling insight into drug mechanisms and metabolic markers of drug efficacy, toxicity and resistance. The present 1H NMR metabolomics study aims to describe the effects of cDDP and Pd2Spm on the polar endometabolome of both MDA-MB-231 cDDP-sensitive and cDDP-resistant cell lines, aiming to describe metabolic markers of (i) resistance upon cDDP treatment, and (ii) the effect of Pd2Spm on the established cDDP-resistant cells. The former observations will give helpful insights about the metabolic features of cDDP-resistance during treatment, and enlighten on the potential role of Pd2Spm in metabolically affecting/tackling cDDP-resistance. |
| Institute | University of Aveiro |
| Department | Department of Chemistry and CICECO-Aveiro Institute of Materials |
| Last Name | Carneiro |
| First Name | Tatiana João |
| Address | Campus Universitário de Santiago, Aveiro, Aveiro, 3810-193, Portugal |
| tatiana.joao@ua.pt | |
| Phone | +351 234 370 200 |
| Submit Date | 2023-11-20 |
| Num Groups | 14 |
| Total Subjects | 126 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2024-05-22 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003101 |
| Sampleprep Summary: | The cellular polar extracts were extracted using a biphasic extraction method of methanol/chloroform/water. Basically, cell pellets were resuspended in 650 µL of 80% (v/v) methanol-miliQ water solution, transferred to microcentrifuge tubes with 150 mg of glass beads, and vortexed for 5 min. Subsequently, 260 µL of 100% chloroform and 260 µL of 100% chloroform plus 220 µL MiliQ water were added to samples, which were vortexed for 5 min between solvents addition. The samples were kept at − 20 °C for 10 min and centrifuged. The aqueous phase of the resulting extract was collected into a new tube, vacuum-dried and stored at − 80 °C until the NMR analysis. All samples and reagents were kept in ice during the extraction procedure. Before NMR analysis, the dry aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)propionic-2,2,3,3-D4 acid sodium salt (TSP-D4) for chemical shift referencing) and transferred into 5 mm NMR tubes. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Biphasic extraction method of methanol/chloroform/water |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Dry aqueous extracts were suspended in 650 µL of 100 mM sodium phosphate buffer (pH 7.4, in D2O containing 0.25% 3-(trimethylsilyl)propionic-2,2,3,3-D4 acid sodium salt (TSP-D4) for chemical shift referencing) |
