Summary of Study ST002996
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001866. The data can be accessed directly via it's Project DOI: 10.21228/M8HM8S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002996 |
Study Title | Tissue Lipidomic Profiling for Detection of Non-Small Cell Lung Cancer |
Study Summary | UPLC-HRMS analysis was performed on AC and SCC patients. OPLSDA classfication was performed on tumor vs. ANT and DNT samples. Panels of discriminant features were identified. The biomarkers identified in discovery set samples for each binary classification were confirmed by using a set of validation samples, which were run separately. Additionally, paired analysis showed the abundance of discriminant compounds has significant altered in tumor tissues compared to corresponding DNT and ANT samples, reflecting the lipid characteristics related to tumor in situ and cancer cell signaling. |
Institute | OUC |
Department | Department of Medicine and Pharmacy |
Laboratory | Analytical chemistry |
Last Name | Zhang |
First Name | Jie |
Address | No. 5 Yushan Road, Qingdao, Shandong, China |
zhangjieqau@163.com | |
Phone | 17806277823 |
Submit Date | 2023-11-16 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-12-27 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP003115 |
Sampleprep Summary: | Frozen tissue samples were thawed on ice. Each tissue sample was cut into small pieces and accurately weighed.To 100 mg tissue sample, 1 mL precooled 80% methanol was added and the sample was homogenized (60 Hz, 90 s) in a tissue homogenizer (Jingxin, JX-24, Shanghai, China). After vortexing for 1 min, the mixture was incubated at -80 °C for 4 hours, followed by centrifuging at 14,000 × g for 10 min at 4 °C. The supernatant was transferred to a new tube. To the rest of the sample, 400 μL dichloromethane/ methanol (3:1, v/v) was added for lipid extraction. The sample mixture was then vortexed twice, each time for 30 s, followed by incubation at -80 °C for 30 min. After homogenization (60 Hz, 90 s) and centrifugation of the sample at 14,000 × g for 10 min at 4 °C, the supernatant was collected and dried under a speed vacuum concentrator. |