Summary of Study ST003006
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001873. The data can be accessed directly via it's Project DOI: 10.21228/M8MF0Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003006 |
| Study Title | BAT metabolomics from cold and TN mouse models |
| Study Summary | Brown adipose tissue (BAT) metabolites in mice acclimated to cold (6˚C) or thermoneutrality (30˚C) for two weeks. N = 10 for mice housed at 6 ˚C and 9 for mice housed at 30 ˚C. |
| Institute | Harvard Medical School |
| Last Name | Wang |
| First Name | Dandan |
| Address | 3 Blackfan Circle |
| dandanwang2022@gmail.com | |
| Phone | 5083733714 |
| Submit Date | 2023-12-13 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-02-19 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP003126 |
| Sampleprep Summary: | For metabolite extraction, tissues were weighed and then homogenized with extraction buffer which consisted of 80% methanol containing Phenylalanine-D₈ internal standard at a 40:1 volume to wet weight ratio. Samples were then centrifuged at 16,000 x g at 4 °C for 15 min. Finally, 50 μL supernatant was transferred to the glass insert for LC-MS detection. |
