Summary of Study ST003017
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001878. The data can be accessed directly via it's Project DOI: 10.21228/M8ZQ63 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003017 |
| Study Title | Lipidomics and plasma hormone reveal indicators of reproductive status in Florida manatees (Trichechus manatus latirostris) |
| Study Summary | Florida manatees (Trichechus manatus latirostris) are protected as a threatened species, and data are lacking regarding their reproductive physiology. This study aimed to (1) quantify plasma steroid hormones in Florida manatees from two field sites, Crystal River and Indian River Lagoon, at different gestational stages and to (2) determine the relationship between plasma progesterone concentrations and lipid biochemistry in relation to pregnancy status. Ultra-high performance liquid chromatography-tandem mass spectrometric analysis was used to measure plasma steroid hormones and lipids. Pregnant female manatees were morphometrically distinct from male and non-pregnant female manatees, characterized by larger body weight and maximal girth. Progesterone concentrations in manatees were also elevated during early gestation versus late gestation. Cholesterol, an important metabolic lipid and precursor for reproductive steroids, was not different between groups. Lipidomics quantified 949 lipids and plasma concentrations of a sphingolipid, ceramide non-hydroxy fatty acid-sphingosine and several glycerophospholipids, including lysophosphatidylcholine, phosphatidylethanolamines, plasmenyl-phosphatidylserines and monomethyl phosphatidylethanolamines, were associated with pregnancy status in the Florida manatee. This research contributes to improving knowledge of manatee reproductive physiology by providing data on plasma steroid hormones relative to reproductive status and by assessing how plasma lipids in healthy Florida manatees correspond to progesterone levels. This lipid panel has potential as a diagnostic approach to identify pregnant individuals in fresh and archived samples. These biochemical and morphometric indicators of reproductive status advance the understanding of manatee physiology. |
| Institute | University of Florida |
| Last Name | Brammer-Robbins |
| First Name | Elizabeth |
| Address | 2187 Mowry Rd., Bldg 471 |
| e.brammerrobbins@ufl.edu | |
| Phone | 9104652899 |
| Submit Date | 2023-12-10 |
| Total Subjects | 57 |
| Num Males | 31 |
| Num Females | 26 |
| Publications | https://doi.org/10.1016/j.ygcen.2023.114250 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003137 |
| Sampleprep Summary: | Lithium heparinized manatee plasma samples, quality control samples, extraction and solvent blanks, and pooled group samples were extracted via the Folch extraction method (2:1 chloroform: methanol) (Folch et al., 1957). The plasma samples were thawed on ice and briefly homogenized. Next, 50 µL of each sample was added to a glass test tube. Three quality control samples were prepared the same manner using 50 µL of SRM 1950. All samples, except the solvent blanks, were spiked with 50 µL of deuterium-labeled internal standards from 10 different lipid classes at known concentrations (diluted 1:10) (Splash Lipidomix, Avanti Polar Lipids, Alabaster, AL). Biphasic extraction was conducted by adding 3 mL of 2:1 chloroform:methanol solution to each test tube including the quality control samples and extraction blanks. The samples were then vortexed for 10 s, 500 µL of Optima-grade water was added, and the tubes were inverted 5 times. All samples were centrifuged at 2500 g for 5 min at 4 °C. The resulting bottom lipid containing layer was then removed and transferred to a new glass test tube using glass Pasteur pipettes. Samples were divided into sub-groups determined by study site, sex, and reproductive status. Extract pools were created by equal contributions from each sample within a group. Finally, the samples, controls, and pools were evaporated under a steady stream of nitrogen at 25 °C for about 25 min. The samples were then reconstituted with 100 µL of isopropanol and vortexed for 10 s. The reconstituted solutions were then transferred to autosampler vial inserts inside glass autosampler vials for UHPLC-MS/MS analysis. |
| Sampleprep Protocol Filename: | Sample Prep.txt |
