Summary of Study ST003155

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001961. The data can be accessed directly via it's Project DOI: 10.21228/M87Q8B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST003155
Study Title	NMR-based metabolomics combined with metabolic pathway analysis reveals metabolic heterogeneity of colorectal cancer tissue at different anatomical locations and stages
Study Summary	Colorectal cancer (CRC) still remains the leading cause of cancer death worldwide. This study aimed to profile the metabolic differences of colorectal cancer tissues (CCT) at different stages and sites, as compared with their adjacent noncancerous tissues (ANT), to investigate the temporal and spatial heterogeneities of metabolic characterization. Our NMR-based metabolomics fingerprinting revealed that many of the metabolite levels were significantly altered in CCT compared to ANT and esophageal cancer tissues, indicating deregulations of glucose metabolism, one-carbon metabolism, glutamine metabolism, amino acid metabolism, fatty acid metabolism, TCA cycle, choline metabolism, etc. A total of five biomarker metabolites, including glucose, glutamate, alanine, valine and histidine, were identified to distinguish between early and advanced stages of CCT. Metabolites that distinguish the different anatomical sites of CCT include glucose, glycerol, glutamine, inositol, succinate, and citrate. Those significant metabolic differences in CRC tissues at different pathological stages and sites suggested temporal and spatial heterogeneities of metabolic characterization in CCT, providing a metabolic foundation for further study on biofluid metabolism in CRC early detection.
Institute	Shantou University Medical College
Department	Radiology Department, Second Affiliated Hospital
Last Name	Lin
First Name	Yan
Address	No. 69, Dongxia North Road, Shantou, Guangdong, China, Shantou, Guangdong, China, 515041, China
Email	994809889@qq.com
Phone	+86 18823992148
Submit Date	2024-03-28
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2024-05-22
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP003279
Sampleprep Summary:	Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.

Sampleprep ID:

SP003279

Sampleprep Summary:

Frozen tissue samples (approximately 300 mg) were thawed at 25°C, then sectioned and homogenized at 16,000 rpm for 80 seconds in a solution consisting of 0.6 mL distilled water and 1.2 mL methanol. Subsequently, chloroform (1.2 mL) and distilled water (1.2 mL) were added and the sample was vortexed for 60 seconds. Finally, samples were incubated on ice for 15 mins, followed by centrifugation at 2000 rpm for 5 mins. The resulting supernatant was washed with nitrogen and evaporated before incubating under vacuum for at least 18 hours. The subsequent lyophilized powder was dissolved in 550 μL PBS/D2O buffer (0.1 M, pH 7.4) and 50 μL TSP/D2O stock solution, and after centrifugation at 10,000 rpm for 5 mins,500 μL of the supernatant was transferred to an NMR tube (5 mm size) for 1H NMR spectroscopy analysis.