Summary of Study ST000001
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000001. The data can be accessed directly via it's Project DOI: 10.21228/M8159B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000001 |
Study Title | Fatb Induction Experiment (FatBIE) |
Study Type | Genotype treatment |
Study Summary | This experiment tests the consequence of a mutation at the FatB gene (At1g08510) in the wound-response of Arabidopsis. The FatB mutant allele (fatb KD J. Ohlrogge (Plant Cell 2003, Vol 15, 1020-1033)) was obtained from Dr. Katayonn Dehesh, University of California, Davis, Davis, CA. This allele is in the Ws background.The standardized growth conditions are as follows: 1. Seeds (between 14 and 16) are sown on media in 100 x 100 x 15mm square Falcon Petri Dishes (Fisher Scientific, catalogue #08-757-11A). Seeds were arranged on the plates in a single horizontal line at the 1-cm mark from the top of the plate.2. Each plate contains between 20 and 25-ml of sterile MS media containing 0.1% (w/v) sucrose.3. Prior to sowing, seeds were sterilized by treating for 1 minute at room temperature with a 300-l solution of 50% (v/v) ethanol, this solution was removed and replaced with a 300-l solution consisting of 1% (v/v) Tween 20 (Fischer BioReagents, catalogue #BP33750), and 50% (v/v) bleach solution (Clorox), and incubated at room temperature for 10-minutes. The seeds were then washed with three changes of 0.3-ml of sterile water. |
Institute | University of California, Davis |
Department | Davis Genome Center |
Laboratory | Fiehn |
Last Name | Kind |
First Name | Tobias |
Address | 451 E. Health Sci. Drive, Davis, CA 95616, USA |
tkind@ucdavis.edu | |
Submit Date | 2013-01-15 |
Num Groups | 4 |
Total Subjects | 24 |
Raw Data Available | No |
Analysis Type Detail | GC-MS |
Release Date | 2013-02-14 |
Release Version | 1 |
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Subject:
Subject ID: | SU000001 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Genotype Strain: | Wassilewskija (Ws) | fatb-ko KD; At1g08510 |
Species Group: | Plant |