Summary of Study ST003071
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001913. The data can be accessed directly via it's Project DOI: 10.21228/M8FH9H This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003071 |
| Study Title | Attenuation of Helicobacter pylori VacA toxin-induced cell death by modulation of intracellular taurine metabolism - Study #4 |
| Study Type | untargeted metabolomics analysis |
| Study Summary | Repletion of intracellular taurine is sufficient to modulate VacA-induced cell death. To determine how supplemental taurine influences cellular metabolism to prevent VacA-induced cell death, we treated both AGS and AZ-521 cells with acid-activated VacA or an acidified buffer control for 12 hours or 5 hours, respectively, in the presence or absence of 10 mM extracellular taurine. Following intoxication, untargeted global metabolomics was conducted on the cell pellets. When the metabolic profiles of VacA-treated cells in the absence of extracellular taurine were compared to the metabolic profiles of VacA-treated cells in the presence of extracellular taurine, taurine was the only metabolite exhibiting significantly different levels in AZ-521 cells and one of two metabolites altered in AGS cells. |
| Institute | Vanderbilt University |
| Department | Chemistry |
| Laboratory | Center for Innovative Technology |
| Last Name | CODREANU |
| First Name | SIMONA |
| Address | 1234 STEVENSON CENTER LANE |
| SIMONA.CODREANU@VANDERBILT.EDU | |
| Phone | 6158758422 |
| Submit Date | 2024-02-07 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-12 |
| Release Version | 1 |
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Subject:
| Subject ID: | SU003186 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | AGS and AZ-521 |
| Species Group: | AGS and AZ-521 |
