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MB Sample ID: SA002826
Local Sample ID: | MR2_AZD_229 |
Subject ID: | SU000074 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | FLT3-ITD AML (MR2) |
Cell Biosource Or Supplier: | Dr. Tim Pardee (Wake Forest Univ.) |
Cell Primary Immortalized: | Primary |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR000075 |
Treatment Summary: | MEK kinase inhibitors |
Treatment Compound: | GSK | AZD |
Treatment Route: | added to media |
Treatment Dose: | 10nM | 10nM |
Treatment Vehicle: | dilutions made in 1X sterile PBS (orginally dissolved in DMSO) |
Cell Storage: | liquid nitrogen, live maintenance in 5% CO2, humidified incubator |
Cell Growth Container: | T-75 culture flask |
Cell Growth Config: | suspension |
Cell Growth Rate: | double ~every 20 hours |
Cell Media: | 50:50 DMEM:IMDM + 10% FBS, 1% P/S |
Cell Harvesting: | Transfer 1 mL to new flask containing 10 mL of fresh culture media |
Cell Pct Confluence: | maintained at ~ 2 x10^6/mL |