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MB Sample ID: SA014119
| Local Sample ID: | L_10_304 |
| Subject ID: | SU000334 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57/BL-6 |
| Gender: | Male |
| Species Group: | Mammal |
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Treatment:
| Treatment ID: | TR000348 |
| Treatment Summary: | Mouse heat stroke model accurately simulates the thermoregulatory, inflammatory, and organ damage responses observed in HS patients. Mice were Surgically implanted with radiotelemetry devices for the continual recording of core temperatrue during heat expsoure and 24 hours of HS recorvery. Mice were orally trated with vehicle and no drug (bacon-flavored treat) or indomethacin (5 mg/kg contained in a bacon-flavored treat) immediately prior to expsoure to a heated chamger (Model 3950, Therma Forma, Marietta, OH; ambient temperature; = 39.5 degrees celsius). Mice remained in the heated chamber, in the absence of food and water, until a maximum of 42.4 degrees celsius was reached. At the maximum temperature mice were removed from the heat and either sacrificed or provided ad libitum food and water until sacrifice at ~3 hours (when mice displayed maximum hypothermia depth; ~30 degrees C) or 24 hours of recovery (24 hrs after the start of heat exposure) when ~1.0 C fever was displayed. Heart, liver, lung, and kidney were rapidly excised, sliced into transverse or longitudinal sections, and fixed in 10% neutral-buffered formalin for histological analysis (Carson Millonig Formulation, Fisher Scientific, Springfield, MA) or stored at -80 degrees celcius for molecular and metabolomics analysis. |
| Treatment Protocol ID: | USARIEM Protocol A1002 - NSAIDS (INDO) |
| Treatment Compound: | Indomethacin |
| Treatment Route: | Oral |
| Treatment Dose: | 5 mg/kg |
| Treatment Vehicle: | Bacon-flavored treat |
| Animal Endp Tissue Coll List: | Heart, Liver, lungs and Kidneys |
