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MB Sample ID: SA023086
Local Sample ID: | M_25 |
Subject ID: | SU000476 |
Subject Type: | Human Cell Media |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Treatment:
Treatment ID: | TR000490 |
Treatment Summary: | Primary cell cultures of desmoid tumor samples were established as previously described [1,2]. Monolayer cell cultures were grown in DMEM supplemented with 5% fetal bovine serum and maintained at 37°C in 5% CO2. Cells were divided when confluent and experiments were performed between the third and sixth passages. Approximately 10 x 106 cells were treated with 1.0uM Dasatinib (Selleck, Houston, USA) dissolved in DMSO, or 0.5uM FAK Inhibitor 14 (Cayman Chemicals Company, Michigan, USA) dissolved in water. Cells were incubated in fresh media containing the inhibitors, or vehicle, for 24 hours. |