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MB Sample ID: SA028878
Local Sample ID: | 400-8 |
Subject ID: | SU000581 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Gender: | Male |
Animal Animal Supplier: | KO: bred and housed at the laboratory animal resource unit (LARU) of North Carolina A&T State University; WT: Charles River Laboratories |
Animal Housing: | group cages of up to 5 mice per cage |
Animal Light Cycle: | 12:12h light:dark cycle |
Animal Feed: | rat chow |
Animal Water: | water ad libitum |
Species Group: | Mammal |
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Treatment:
Treatment ID: | TR000595 |
Treatment Summary: | Type 1 diabetes was induced at age 8 weeks. The mice were fasted for 6 hours prior to being injected with low dose STZ (50 mg/kg body weight) in sodium citrate buffer (10 mmol/L, pH 4.5) daily for 5 days following the protocols described by Tesch and Allen and recommended by the Animal Models of Diabetic Complications Consortium (AMDCC; amdcc.org)1. Control mice were injected with equivalent volumes of the sodium citrate buffer. Diabetes was confirmed by measuring fasting blood glucose levels using a standard glucose meter at 10 days post-STZ injection. Mice with a fasting glucose level ≥280 mg/dL were considered diabetic. STZ-injected mice with a fasting blood glucose of <280 mg/dL (15mmol/L) were culled and eliminated from the study. |
Treatment Compound: | streptozotocin |
Treatment Dose: | 50 mg/kg body weight |
Treatment Doseduration: | daily; 5 days |
Treatment Vehicle: | sodium citrate |
Animal Endp Tissue Coll List: | plasma, urine, kidney tissue |