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MB Sample ID: SA035015
| Local Sample ID: | CD06241620 |
| Subject ID: | SU000644 |
| Subject Type: | Photosynthetic organism |
| Subject Species: | Chlamydomonas reinhardtii |
| Taxonomy ID: | 3055 |
| Genotype Strain: | CC125 Wild type |
| Species Group: | Microorganism |
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Treatment:
| Treatment ID: | TR000658 |
| Treatment Summary: | For compound treatment, cells from mid-log phase culture were harvested, washed once with fresh sterile TAP media and inoculated at starting density of 5 x 105 cells/mL. Thus for the current experiment 3 different treatment conditions were generated. Cells without compound treatment were negative control for the lipid accumulation and 2 compounds were used to generate treatment conditions. All compounds were used at a final concentration of 5 M (this concentration was determined using a dose-response curve). For all cultures, 250 mL Erlenmeyer flasks with rubber stopper adopted to facilitate gas exchange were used and maintained in horizontal orbital shaking growing chamber (Innova 43; New Brunswick). At the end of 72h, cells were harvested, media was removed via centrifugation and biomass was stored at -80 C until metabolite extraction. |
| Cell Media: | Tris-Acetate Phoshphate (TAP) media. |
