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MB Sample ID: SA045316
Local Sample ID: | Tgon_HKKO_13Cglutamine_t000_B |
Subject ID: | SU000843 |
Subject Type: | Cells (protozoan parasite) |
Subject Species: | Toxoplasma gondii |
Taxonomy ID: | 5811 |
Genotype Strain: | RH (Type I strain) |
Cell Biosource Or Supplier: | ATCC |
Subject Comments: | Asexual stage (Tachyzoite) |
Cell Passage Number: | In continuous culture |
Cell Counts: | 10^8 cells per sample |
Species Group: | Microorganism |
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Treatment:
Treatment ID: | TR000857 |
Treatment Summary: | Tachyzoite stage Toxoplasma gondii parasites were freshly isolated from infected Human Foreskin Fibroblast monolayers and the extracellular parasites were incubated in DMEM medium supplemented with either +13C6-glucose or +13C5-15N2-glutamine. Treatment with labeled substrates was done for 0, 5, 10, 30, 60 & 120 minutes before harvesting and processing the cells for LC-MS analysis. |
Cell Storage: | Liquid nitrogen cell storage system |
Cell Growth Container: | Standard incubator maintaining 37 deg. Cel. & 5% CO2 |
Cell Media: | DMEM minus glucose medium (cat. No. 11966; Gibco, ThermoFisher Scientific) supplemented with +13C6-glucose. DMEM minus glutamine medium (cat. No. 11960; Gibco, ThermoFisher Scientific) supplemented with +13C5-15N2-glutamine. |