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MB Sample ID: SA045321
| Local Sample ID: | Tgon_RH_13Cglucose_t010_B |
| Subject ID: | SU000843 |
| Subject Type: | Cells (protozoan parasite) |
| Subject Species: | Toxoplasma gondii |
| Taxonomy ID: | 5811 |
| Genotype Strain: | RH (Type I strain) |
| Cell Biosource Or Supplier: | ATCC |
| Subject Comments: | Asexual stage (Tachyzoite) |
| Cell Passage Number: | In continuous culture |
| Cell Counts: | 10^8 cells per sample |
| Species Group: | Microorganism |
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Treatment:
| Treatment ID: | TR000857 |
| Treatment Summary: | Tachyzoite stage Toxoplasma gondii parasites were freshly isolated from infected Human Foreskin Fibroblast monolayers and the extracellular parasites were incubated in DMEM medium supplemented with either +13C6-glucose or +13C5-15N2-glutamine. Treatment with labeled substrates was done for 0, 5, 10, 30, 60 & 120 minutes before harvesting and processing the cells for LC-MS analysis. |
| Cell Storage: | Liquid nitrogen cell storage system |
| Cell Growth Container: | Standard incubator maintaining 37 deg. Cel. & 5% CO2 |
| Cell Media: | DMEM minus glucose medium (cat. No. 11966; Gibco, ThermoFisher Scientific) supplemented with +13C6-glucose. DMEM minus glutamine medium (cat. No. 11960; Gibco, ThermoFisher Scientific) supplemented with +13C5-15N2-glutamine. |
