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MB Sample ID: SA052772
| Local Sample ID: | Control_2.3 |
| Subject ID: | SU000935 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Daoy (ATCC® HTB-186™) |
| Subject Comments: | cancer cell line |
| Species Group: | Human |
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Treatment:
| Treatment ID: | TR000949 |
| Treatment Summary: | siRNA constructs targeting p73 (ID: 2671-AMBION) and a non-targeting control siRNA (scramble) were transfected with 10 pM siRNA with lipofectamine 3000 according to the supplier’s protocol for 48 hours |
| Cell Growth Container: | plate |
| Cell Growth Config: | attached |
| Cell Growth Rate: | 36 hours |
| Cell Inoc Proc: | lipofectamine 3000 |
| Cell Media: | DMEM + GlutaMAX medium (Gibco), supplemented with 10% v/v foetal bovine serum (FBS, Gibco) and penicillin/streptomycin (1 U/ml, Gibco), |
| Cell Envir Cond: | 37°C in humidified 5% CO2 |
| Cell Harvesting: | After 48 h of transfection the cells were washed three times with ice-cold PBS (3x5 mL) and the celles were collected using a cell scraper. Ice-cold water (3 mL,sterile, MilliQ) was added to the samples. The suspension was transferred to a 15 mL polypropylene tube and then snap-frozen in liquid N2 and stored on ice for 5 min. The cells were lysed by two freeze-thaw cycles where the cells were thawed at 37°C in a water bath and frozen using liquid N2. Subsequently, the cells were sonicated in a |
| Cell Pct Confluence: | 80-90% |
| Cell Media Lastchanged: | 48 hours |
