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MB Sample ID: SA052779
Local Sample ID: | Control_1.3 |
Subject ID: | SU000935 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | ATCC |
Cell Strain Details: | Daoy (ATCC® HTB-186™) |
Subject Comments: | cancer cell line |
Species Group: | Human |
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Treatment:
Treatment ID: | TR000949 |
Treatment Summary: | siRNA constructs targeting p73 (ID: 2671-AMBION) and a non-targeting control siRNA (scramble) were transfected with 10 pM siRNA with lipofectamine 3000 according to the supplier’s protocol for 48 hours |
Cell Growth Container: | plate |
Cell Growth Config: | attached |
Cell Growth Rate: | 36 hours |
Cell Inoc Proc: | lipofectamine 3000 |
Cell Media: | DMEM + GlutaMAX medium (Gibco), supplemented with 10% v/v foetal bovine serum (FBS, Gibco) and penicillin/streptomycin (1 U/ml, Gibco), |
Cell Envir Cond: | 37°C in humidified 5% CO2 |
Cell Harvesting: | After 48 h of transfection the cells were washed three times with ice-cold PBS (3x5 mL) and the celles were collected using a cell scraper. Ice-cold water (3 mL,sterile, MilliQ) was added to the samples. The suspension was transferred to a 15 mL polypropylene tube and then snap-frozen in liquid N2 and stored on ice for 5 min. The cells were lysed by two freeze-thaw cycles where the cells were thawed at 37°C in a water bath and frozen using liquid N2. Subsequently, the cells were sonicated in a |
Cell Pct Confluence: | 80-90% |
Cell Media Lastchanged: | 48 hours |