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MB Sample ID: SA076764
Local Sample ID: | 6-Early Exponential Phase |
Subject ID: | SU001169 |
Subject Type: | Bacteria |
Subject Species: | Acidithiobacillus ferrooxidans ATCC 23270 |
Taxonomy ID: | 243159 |
Genotype Strain: | ATCC 23270 |
Cell Biosource Or Supplier: | American Type Culture Collection |
Cell Strain Details: | ATCC 23270 |
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Treatment:
Treatment ID: | TR001183 |
Treatment Summary: | After the cells were concentrated into a 1.5ml centrifuge tube, the cellular metabolism was quenched using 40ul of 60% MeOH and 40% H2O (v/v) with 0.85% (w/v) of Ammonium Formate, adjusted to pH 2.5 and cooled to -20 °C. The quenched cells were centrifuged for 5 minutes at 2,319 x g at 4 °C. The cells were then placed on dry ice, the cell supernatant was removed and an isopropanol:methanol:water (IMW) (3:3:2) extraction solution that was cooled to -20 °C was added. These solutions were then vigorously vortexed and then centrifuged at 15,682 x g for 10 min. The extraction liquid was then collected and placed at -80 °C until further analysis. |