Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA076774

Local Sample ID:	10-Late Exponential Phase
Subject ID:	SU001169
Subject Type:	Bacteria
Subject Species:	Acidithiobacillus ferrooxidans ATCC 23270
Taxonomy ID:	243159
Genotype Strain:	ATCC 23270
Cell Biosource Or Supplier:	American Type Culture Collection
Cell Strain Details:	ATCC 23270

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Treatment:

Treatment ID:	TR001183
Treatment Summary:	After the cells were concentrated into a 1.5ml centrifuge tube, the cellular metabolism was quenched using 40ul of 60% MeOH and 40% H2O (v/v) with 0.85% (w/v) of Ammonium Formate, adjusted to pH 2.5 and cooled to -20 °C. The quenched cells were centrifuged for 5 minutes at 2,319 x g at 4 °C. The cells were then placed on dry ice, the cell supernatant was removed and an isopropanol:methanol:water (IMW) (3:3:2) extraction solution that was cooled to -20 °C was added. These solutions were then vigorously vortexed and then centrifuged at 15,682 x g for 10 min. The extraction liquid was then collected and placed at -80 °C until further analysis.

Treatment ID:

TR001183

Treatment Summary:

After the cells were concentrated into a 1.5ml centrifuge tube, the cellular metabolism was quenched using 40ul of 60% MeOH and 40% H2O (v/v) with 0.85% (w/v) of Ammonium Formate, adjusted to pH 2.5 and cooled to -20 °C. The quenched cells were centrifuged for 5 minutes at 2,319 x g at 4 °C. The cells were then placed on dry ice, the cell supernatant was removed and an isopropanol:methanol:water (IMW) (3:3:2) extraction solution that was cooled to -20 °C was added. These solutions were then vigorously vortexed and then centrifuged at 15,682 x g for 10 min. The extraction liquid was then collected and placed at -80 °C until further analysis.