Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA077641

Local Sample ID:	33
Subject ID:	SU001174
Subject Type:	NMR based metabolomics of microbes
Subject Species:	Nesterenkonia flava
Taxonomy ID:	469799

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Treatment
Sample Preparation
Analysis
NMR

Treatment:

Treatment ID:	TR001188
Treatment Summary:	For each cell pellet sample, 5 mL of the mixture of methanol/water (1:0.9, v/v) was added and ultrasonicated for 25 min to break cells bathing in ice. The mixture was added with 5 mL of cold chloroform, vortexed and subjected to 10 min of ultrasonic extraction under the bath of ice. The mixture was then centrifuged at 9500 g for 8 min. The upper layer phase containing methanol and water was taken out and evaporated in the fume cupboard to remove methanol. The remaining water solution was freezed under −80 °C, and then freeze-dried to afford dry samples, which were stored under −80 °C before analysis.

Treatment ID:

TR001188

Treatment Summary:

For each cell pellet sample, 5 mL of the mixture of methanol/water (1:0.9, v/v) was added and ultrasonicated for 25 min to break cells bathing in ice. The mixture was added with 5 mL of cold chloroform, vortexed and subjected to 10 min of ultrasonic extraction under the bath of ice. The mixture was then centrifuged at 9500 g for 8 min. The upper layer phase containing methanol and water was taken out and evaporated in the fume cupboard to remove methanol. The remaining water solution was freezed under −80 °C, and then freeze-dried to afford dry samples, which were stored under −80 °C before analysis.