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MB Sample ID: SA078071
| Local Sample ID: | MM2 |
| Subject ID: | SU001189 |
| Subject Type: | Bacteria |
| Subject Species: | Bacteroides thetaiotaomicron VPI-5482 |
| Taxonomy ID: | 226186 |
| Genotype Strain: | WT (VPI-5482), SPT K.O. (BT_0870) |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Wild type Bacteroides tethaiotamicron strains and serine palmitoyltransferase (SPT) knockouts |
| Subject Comments: | Grown in Minimal Media with or without d4-alanine |
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Treatment:
| Treatment ID: | TR001204 |
| Treatment Summary: | WT and ΔSPT cultures of B. thetaiotaomicron and B. ovatus were grown in 5 mL of minimal media (M9 salts (Teknova), 1% vitamin K1-hemin solution (Becton Dickinson), 1% trace mineral supplement (ATCC), 1% trace vitamin supplement (ATCC), 2% lactose), supplemented with 10μM of deuterium (D4)-labelled alanine (Sigma), or 10μM of -alanine (Sigma). |
| Cell Storage: | -80C |
| Cell Growth Container: | anaerobic chamber (Coy Laboratory Products) with an atmosphere of 20% CO2, 5% H2, and 75% N2 at 37°C. |
