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MB Sample ID: SA079821
Local Sample ID: | ATQ-20180530 |
Subject ID: | SU001214 |
Subject Type: | Cultured cells |
Subject Species: | Plasmodium falciparum |
Taxonomy ID: | 5833 |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR001229 |
Treatment Summary: | Infected Red Blood cells were exposed to Antimalarial Compounds, associated with sample names. All samples were treated with 10xIC50 of a compound for 2.5 hours or left untreated for 2.5 hours. |
Treatment Protocol ID: | Metabolite_Extraction_for_LCMS_2017_Llinas |
Treatment Protocol Filename: | Metabolite_Extraction_for_LCMS_2017_Llinas.pdf |
Treatment: | Abiotic |
Treatment Compound: | ATQ, MMV009108, MMV019662, MMV028038 |
Treatment Route: | Addition to media |
Treatment Dose: | 10xIC50, 2-10xIC50 for archival data |
Treatment Dosevolume: | Always equal to or less than 10 uL per 5 mL sample |
Treatment Doseduration: | 2.5 hours |
Treatment Vehicle: | Stocks kept in either DMSO or Water |
Cell Storage: | Temperature/Gas Mixture controlled incubator |
Cell Growth Container: | 6-well plate |
Cell Growth Config: | 5 mL per well, each with 1x10^8 cells |
Cell Media: | RPMI 1640 w/ Albumax, Gentamycin, Hypoxanthine, HEPES, Sodium Bicarbonate |
Cell Harvesting: | PBS Wash followed by 90% methanol extraction |