Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA086276

Local Sample ID:	BBSe-1
Subject ID:	SU001288
Subject Type:	Invertebrate
Subject Species:	Bombus impatiens
Taxonomy ID:	132113

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Treatment:

Treatment ID:	TR001303
Treatment Summary:	We obtained five commercial Bombus impatiens colonies that each contained approximately 50 workers, a gravid queen, pollen and a Biogluc® feeder from the Biobest Group (Biobest USA Inc., Romulus, MI). We immediately replaced the Biogluc with sterile 60% sucrose and allowed the bees access ad libitum. We also provided the colony with pollen patties ad libitum and kept the colonies at 29 °C under constant darkness at the University of California, Riverside. We allowed the colonies to grow undisturbed for two weeks before starting the experiment. Once the colonies had populated, we transferred 10 bees (two cohorts of five) to 475 mL polypropylene containers (WebstaurantStore, Lancaster, PA) and reared them at 29 °C under 24-hour darkness. We then exposed the bees to treatments consisting of 60% sucrose spiked with 0.6 mg/kg sodium selenate and used unspiked 60% sucrose as a control.

Treatment ID:

TR001303

Treatment Summary:

We obtained five commercial Bombus impatiens colonies that each contained approximately 50 workers, a gravid queen, pollen and a Biogluc® feeder from the Biobest Group (Biobest USA Inc., Romulus, MI). We immediately replaced the Biogluc with sterile 60% sucrose and allowed the bees access ad libitum. We also provided the colony with pollen patties ad libitum and kept the colonies at 29 °C under constant darkness at the University of California, Riverside. We allowed the colonies to grow undisturbed for two weeks before starting the experiment. Once the colonies had populated, we transferred 10 bees (two cohorts of five) to 475 mL polypropylene containers (WebstaurantStore, Lancaster, PA) and reared them at 29 °C under 24-hour darkness. We then exposed the bees to treatments consisting of 60% sucrose spiked with 0.6 mg/kg sodium selenate and used unspiked 60% sucrose as a control.