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MB Sample ID: SA090776
Local Sample ID: | SC_20180803_RPLCp_DDA2_Sup_QC_06 |
Subject ID: | SU001312 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Gender: | Not applicable |
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Treatment:
Treatment ID: | TR001327 |
Treatment Summary: | For each isolate, a single colony from an agar dish was inoculated in 5 mL LB and shaken overnight at 37°C under ambient atmospheric conditions. Cultures were then diluted 1:1000 in the combined human urine and grown for 6 hours to mid-log phase (37°C, shaking), under 4% oxygen to emulate the bladder environment. After 6 hours, OD600 of each isolate was measured – and cultures were normalized by volume to yield equal number of organisms from each strain – prior to pooling into groups of 8 isolates each. CFUs were enumerated for each pool to confirm bacterial denisty (~109 total E. coli per pool). Each pool was then centrifuged to separate the cellular (pellet) and supernatant fraction. Pellets and supernatants were flash frozen and stored at -80°C until for metabolomic analysis. |