Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA094158

Local Sample ID:	10_18_2011 M1A
Subject ID:	SU001370
Subject Type:	Plant
Subject Species:	Corylus avellana;Corylus americana
Taxonomy ID:	13451;78632
Genotype Strain:	Corylus avellana;Corylus americana(cv Geneva)

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Treatment:

Treatment ID:	TR001385
Treatment Summary:	Filbert (C. avellana L. × C. americana Marshall cv. Geneva; Grimo Nut Nursery, Niagara-on-the-Lake, ON, Canada) shoot cultures were provided from germplasm maintained at the Gosling Research Institute for Plant Preservation (GRIPP; University of Guelph, Guelph, ON). Plantlets were grown in GA-7 vessels according to methods previously described (Garrison et al. 2013; Latawa et al. 2016). In brief, cultures were grown on semi-solid modified NCGR-COR medium (Yu and Reed 1995) supplemented with 10 g L-1 myo-inositol, 200 mg L-1 glycine, 100 mg L-1 nicotinic acid, 100 mg L-1 thiamine (PhytoTechnology Laboratories), 17.6 µM benzylaminopurine (BAP; Sigma-Aldrich), 0.014 µM indole-3-butyric acid (IBA; Sigma-Aldrich), 0.29 µM gibberellic acid (GA3; PhytoTechnology Laboratories), and 30 g L-1 glucose with 0, 230, 460 and 690 µM Fe-EDDHA (Sigma, St. Louis, MO) and the pH of the medium was adjusted to 5.7 before autoclaving at 121 ºC for 20 min. Cultures were maintained in a growth room at 23 ± 2oC with a 16 h photoperiod of 40 µmol m-2 s-1 provided by cool-white fluorescent lamps (Osram Sylvania Ltd., Mississauga, ON, Canada).

Treatment ID:

TR001385

Treatment Summary:

Filbert (C. avellana L. × C. americana Marshall cv. Geneva; Grimo Nut Nursery, Niagara-on-the-Lake, ON, Canada) shoot cultures were provided from germplasm maintained at the Gosling Research Institute for Plant Preservation (GRIPP; University of Guelph, Guelph, ON). Plantlets were grown in GA-7 vessels according to methods previously described (Garrison et al. 2013; Latawa et al. 2016). In brief, cultures were grown on semi-solid modified NCGR-COR medium (Yu and Reed 1995) supplemented with 10 g L-1 myo-inositol, 200 mg L-1 glycine, 100 mg L-1 nicotinic acid, 100 mg L-1 thiamine (PhytoTechnology Laboratories), 17.6 µM benzylaminopurine (BAP; Sigma-Aldrich), 0.014 µM indole-3-butyric acid (IBA; Sigma-Aldrich), 0.29 µM gibberellic acid (GA3; PhytoTechnology Laboratories), and 30 g L-1 glucose with 0, 230, 460 and 690 µM Fe-EDDHA (Sigma, St. Louis, MO) and the pH of the medium was adjusted to 5.7 before autoclaving at 121 ºC for 20 min. Cultures were maintained in a growth room at 23 ± 2oC with a 16 h photoperiod of 40 µmol m-2 s-1 provided by cool-white fluorescent lamps (Osram Sylvania Ltd., Mississauga, ON, Canada).