Return to study ST001622 main page
MB Sample ID: SA137925
Local Sample ID: | Fru1 |
Subject ID: | SU001699 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Female |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR001712 |
Treatment Summary: | For metabolic flux analysis of 13C-labeled glucose, intestinal organoids were pretreated with unlabeled fructose (10 mM) for 24 hr, washed with PBS, and then switched into 13C labeled glucose-containing DMEM/F12 medium (10 mM U-[13C] D-glucose, Cambridge Isotope Laboratories) for 6 hr. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr. |