Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA152223

Local Sample ID:	218
Subject ID:	SU001742
Subject Type:	Mammal
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Age Or Age Range:	10 weeks
Gender:	Male

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Treatment:

Treatment ID:	TR001755
Treatment Summary:	All heart perfusions underwent an initial 15-minute equilibration period with Krebs Ringer bicarbonate buffer containing 119 mM NaCl, 4.8 mM KCl, 2.6 mM CaCl2, 1.2 mM KH2PO4, 1.2 mM MgSO4, 25 mM NaHCO3, 11 mM glucose, and 0.05 mM L-carnitine at a flow rate of 12 ml/minute. After the equilibrium period, hearts were perfused for 30 minutes with 3% BSA, 100 µU/mL insulin, 0.4 mM palmitate, physiologic concentrations of amino acids, and either DMSO (Veh), BT2, or LY3351337.

Treatment ID:

TR001755

Treatment Summary:

All heart perfusions underwent an initial 15-minute equilibration period with Krebs Ringer bicarbonate buffer containing 119 mM NaCl, 4.8 mM KCl, 2.6 mM CaCl2, 1.2 mM KH2PO4, 1.2 mM MgSO4, 25 mM NaHCO3, 11 mM glucose, and 0.05 mM L-carnitine at a flow rate of 12 ml/minute. After the equilibrium period, hearts were perfused for 30 minutes with 3% BSA, 100 µU/mL insulin, 0.4 mM palmitate, physiologic concentrations of amino acids, and either DMSO (Veh), BT2, or LY3351337.